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rnase r treated fraction  (TaKaRa)


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    Structured Review

    TaKaRa rnase r treated fraction
    Identification and expression analysis of circMEF2C(2, 3) (A) Diagram illustrating the generation of circMEF2C(2, 3) from the MEF2C gene. (B) Detection of circMEF2C(2, 3) in cDNA and gDNA using divergent and convergent primers via PCR. (C) Detection of circMEF2C(2, 3) and MEF2C expression after <t>RNase</t> R digestion (37°C, 50 min). (D) Confirmation of the back-splice junction (BSJ) of circMEF2C(2, 3) through Sanger sequencing. (E) Subcellular localization analysis of circMEF2C(2, 3). (F) Tissue expression profile of circMEF2C(2, 3). (G) Expression patterns of circMEF2C(2, 3) during adipogenesis. (H) Expression of circMEF2C(2, 3) in adipose tissue of MS pigs and DLY pigs. (I) Expression of circMEF2C(2, 3) in muscle tissue of MS pigs and DLY pigs.
    Rnase R Treated Fraction, supplied by TaKaRa, used in various techniques. Bioz Stars score: 97/100, based on 3731 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rnase+r+treated+fraction/RNase+A/pmc11059125-226-6-15
    Average 97 stars, based on 3731 article reviews
    rnase r treated fraction - by Bioz Stars, 2026-09
    97/100 stars

    Images

    1) Product Images from "CircMEF2C(2, 3) modulates proliferation and adipogenesis of porcine intramuscular preadipocytes by miR-383/671-3p/ MEF2C axis"

    Article Title: CircMEF2C(2, 3) modulates proliferation and adipogenesis of porcine intramuscular preadipocytes by miR-383/671-3p/ MEF2C axis

    Journal: iScience

    doi: 10.1016/j.isci.2024.109710

    Identification and expression analysis of circMEF2C(2, 3) (A) Diagram illustrating the generation of circMEF2C(2, 3) from the MEF2C gene. (B) Detection of circMEF2C(2, 3) in cDNA and gDNA using divergent and convergent primers via PCR. (C) Detection of circMEF2C(2, 3) and MEF2C expression after RNase R digestion (37°C, 50 min). (D) Confirmation of the back-splice junction (BSJ) of circMEF2C(2, 3) through Sanger sequencing. (E) Subcellular localization analysis of circMEF2C(2, 3). (F) Tissue expression profile of circMEF2C(2, 3). (G) Expression patterns of circMEF2C(2, 3) during adipogenesis. (H) Expression of circMEF2C(2, 3) in adipose tissue of MS pigs and DLY pigs. (I) Expression of circMEF2C(2, 3) in muscle tissue of MS pigs and DLY pigs.
    Figure Legend Snippet: Identification and expression analysis of circMEF2C(2, 3) (A) Diagram illustrating the generation of circMEF2C(2, 3) from the MEF2C gene. (B) Detection of circMEF2C(2, 3) in cDNA and gDNA using divergent and convergent primers via PCR. (C) Detection of circMEF2C(2, 3) and MEF2C expression after RNase R digestion (37°C, 50 min). (D) Confirmation of the back-splice junction (BSJ) of circMEF2C(2, 3) through Sanger sequencing. (E) Subcellular localization analysis of circMEF2C(2, 3). (F) Tissue expression profile of circMEF2C(2, 3). (G) Expression patterns of circMEF2C(2, 3) during adipogenesis. (H) Expression of circMEF2C(2, 3) in adipose tissue of MS pigs and DLY pigs. (I) Expression of circMEF2C(2, 3) in muscle tissue of MS pigs and DLY pigs.

    Techniques Used: Expressing, Sequencing


    Figure Legend Snippet:

    Techniques Used: Recombinant, Real-time Polymerase Chain Reaction, Isolation, Negative Control, Software

    Related Articles

    Synthesized:

    Article Title: CircMEF2C(2, 3) modulates proliferation and adipogenesis of porcine intramuscular preadipocytes by miR-383/671-3p/ MEF2C axis
    Article Snippet: .. Subsequently, cDNA was synthesized from this RNase R-treated fraction using the PrimeScript RT Master kit (Takara). .. Total RNA was extracted from the cells using Trizol reagent kit (Thermo Fisher Scientific).



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    rnase r treated fraction  (TaKaRa)
    97
    TaKaRa rnase r treated fraction
    Identification and expression analysis of circMEF2C(2, 3) (A) Diagram illustrating the generation of circMEF2C(2, 3) from the MEF2C gene. (B) Detection of circMEF2C(2, 3) in cDNA and gDNA using divergent and convergent primers via PCR. (C) Detection of circMEF2C(2, 3) and MEF2C expression after <t>RNase</t> R digestion (37°C, 50 min). (D) Confirmation of the back-splice junction (BSJ) of circMEF2C(2, 3) through Sanger sequencing. (E) Subcellular localization analysis of circMEF2C(2, 3). (F) Tissue expression profile of circMEF2C(2, 3). (G) Expression patterns of circMEF2C(2, 3) during adipogenesis. (H) Expression of circMEF2C(2, 3) in adipose tissue of MS pigs and DLY pigs. (I) Expression of circMEF2C(2, 3) in muscle tissue of MS pigs and DLY pigs.
    Rnase R Treated Fraction, supplied by TaKaRa, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rnase+r+treated+fraction/RNase+A/pmc11059125-226-6-15
    Average 97 stars, based on 1 article reviews
    rnase r treated fraction - by Bioz Stars, 2026-09
    97/100 stars
    		  Buy from Supplier

    Image Search Results


    Identification and expression analysis of circMEF2C(2, 3) (A) Diagram illustrating the generation of circMEF2C(2, 3) from the MEF2C gene. (B) Detection of circMEF2C(2, 3) in cDNA and gDNA using divergent and convergent primers via PCR. (C) Detection of circMEF2C(2, 3) and MEF2C expression after RNase R digestion (37°C, 50 min). (D) Confirmation of the back-splice junction (BSJ) of circMEF2C(2, 3) through Sanger sequencing. (E) Subcellular localization analysis of circMEF2C(2, 3). (F) Tissue expression profile of circMEF2C(2, 3). (G) Expression patterns of circMEF2C(2, 3) during adipogenesis. (H) Expression of circMEF2C(2, 3) in adipose tissue of MS pigs and DLY pigs. (I) Expression of circMEF2C(2, 3) in muscle tissue of MS pigs and DLY pigs.

    Journal: iScience

    Article Title: CircMEF2C(2, 3) modulates proliferation and adipogenesis of porcine intramuscular preadipocytes by miR-383/671-3p/ MEF2C axis

    doi: 10.1016/j.isci.2024.109710

    Figure Lengend Snippet: Identification and expression analysis of circMEF2C(2, 3) (A) Diagram illustrating the generation of circMEF2C(2, 3) from the MEF2C gene. (B) Detection of circMEF2C(2, 3) in cDNA and gDNA using divergent and convergent primers via PCR. (C) Detection of circMEF2C(2, 3) and MEF2C expression after RNase R digestion (37°C, 50 min). (D) Confirmation of the back-splice junction (BSJ) of circMEF2C(2, 3) through Sanger sequencing. (E) Subcellular localization analysis of circMEF2C(2, 3). (F) Tissue expression profile of circMEF2C(2, 3). (G) Expression patterns of circMEF2C(2, 3) during adipogenesis. (H) Expression of circMEF2C(2, 3) in adipose tissue of MS pigs and DLY pigs. (I) Expression of circMEF2C(2, 3) in muscle tissue of MS pigs and DLY pigs.

    Article Snippet: Subsequently, cDNA was synthesized from this RNase R-treated fraction using the PrimeScript RT Master kit (Takara).

    Techniques: Expressing, Sequencing

    Journal: iScience

    Article Title: CircMEF2C(2, 3) modulates proliferation and adipogenesis of porcine intramuscular preadipocytes by miR-383/671-3p/ MEF2C axis

    doi: 10.1016/j.isci.2024.109710

    Figure Lengend Snippet:

    Article Snippet: Subsequently, cDNA was synthesized from this RNase R-treated fraction using the PrimeScript RT Master kit (Takara).

    Techniques: Recombinant, Real-time Polymerase Chain Reaction, Isolation, Negative Control, Software